Isolation of bacteriophage from sewage, Pond, Lake Water against MDR Escherichia coli

Abstract

Background: The prevalence of highly resistant pathogens has created significant challenges in the treatment of patients, particularly within hospital settings. The practice of phage therapy, which uses bacterial viruses (phages) to treat bacterial infections, has been around for almost a century. The universal decline in the effectiveness of antibiotics has generated renewed interest in revisiting this practice. Method: he study was conducted at the Department of Microbiology, RKDF Medical College Hospital & Research Centre, SRK University, located in Bhopal, Madhya Pradesh. Results: We included total 140 cases. Among the 140 Escherichia coli, 122 were MDR Escherichia coli and rest were normal E. coli. After that, Bacteriophages were prepared against 122 MDR Escherichia Coli which were isolated from sewage pond lake water and see the in vitro lytic efficacy of bacteriophages for their presence. So the presence of bacteriophage is more in sewage water as compared to other water samples. Conclusion: In conclusion, it may be suggested bacteriophage can be used as a therapy and phage therapy might be beneficial for the patient   who are suffering with the MDR strains from a long period of time.