Assessment Of ImmunohistochemicalexpressionOfmt1mmp/MMP14 In Oral Submucous Fibrosis And Oral Submucous Fibrosis With Concomitant Oral Squamous Cell Carcinoma

Abstract

Abstract:

Background : Recently  recognized as “collagen metabolic disorder” also a well known oral potentially malignant disorder with  about 7-13% malignant transformation rate, “Oral submucous fibrosis” (OSF) is incessant, unabating, ineradicable disease. From last few years researches have been conducted to study different molecular aspects of carcinogenesis in OSF through various appurtenant and supplemental methods which is important from  clinical as well as the histological point of view. Ultimately targeting these molecules through tailored treatment modalities and finally improving overall survival of high risk cases of OSF patients.

Material and Methods:We have taken 25 cases each of “OSF .” and OSF with concomitant Oral Squamous Cell Carcinoma along with 25 cases of normal mucosa. Immunohistochemical staining of 4 μm thick sections of formalin fixed paraffin embedded blocks with Polyclonal rabbit MT-1 MMP antibody was done in each case All the IHC slides were blinded for analysis by two independent experienced oral pathologists for scoring of these antibodies

Results:one way ANOVA. chi square test and Mann Whitney U test. Highly statistically significant difference when comparison was made between OSF, OSF with OSCC and control groups with ( p = 0.000) A statistically non-significant differences was found  in the expression of stromal cells  between OSF and  OSF with OSCC cases. (with p= 0.090)

Conclusion The semiquantitative expression of MT1MMP could assess early invasion in high risk cases of OSF even before its clinical appearance.

Keywords: MT-1 MMP,Oral submucous fibrosis, malignant potential in OSF