Attenuating Potential of Modified Diets on Testicular Inflammatory Biomarkers in Streptozotocin-Induced Diabetic Wistar Rats

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OSSAI Nduka Richard
NWANGWA Eze Kingsley
OJIEH Anthony Emeka
NWOGUEZE Bartholomew Chukwuebuka


In vascularized tissues, inflammation is a complex and dynamic defensive response to cell injury, infection by bacteria, trauma, or toxins. Inflammatory biomarkers are molecules that regulate the inflammatory response in all immune system cells. Numerous mediators are released from the attack site, and different host immune system cells infiltrate the area during inflammation. Without the combination of regulated leukocyte population migration, different inflammatory mediators, inflammatory biomarkers (acute or systemic inflammatory marker), and subsequent physiologic changes that carry inflammatory responses, it would be impossible to assemble and regulate inflammatory responses. This study focuses on the attenuating potential of modified diets on testicular inflammatory biomarkers in streptozotocin-induced diabetic Wistar rats. Ninety-six adults male Wistar rats were divided into four units, of four groups/unit and each group consisting of six rats. Unit 1 is non-diabetic unit, whereas Units 2, 3, and 4 were induced with type 2 diabetes. While the rats in group one in all the units were fed with standard rat chaw, group 2 received HFD, group three received HPD, while groups four received HCD. Alpha lipoic acid (200 mg/kg body weight) and metformin (50 mg/kg body weight) were also given to the rats in units three and four, respectively. After 12 weeks of treatment and feeding, each rat was euthanized and testes were excised for biochemical analysis. Data were examined using GraphPad Prism. Two-way analysis of variance (ANOVA) was used to examine the mean variations between groups. The Tukey post hoc test was then performed, with p-values of 0.05 being deemed statistically significant. According to the current study's findings, high-protein meals, both by themselves and in conjunction with ALA, reduce blood glucose levels and the inflammatory damage brought on by streptozotocin toxicity. For this reason, they may be utilized to treat diabetes mellitus and autoimmune-induced inflammation.

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